Journal: bioRxiv

Article Title: Prospective isolation according to melanin pigment content of melanoma cells with heterogeneous potentials for disease propagation

doi: 10.1101/2022.10.31.514484

Figure Lengend Snippet: Flow cytometric analysis of LP (blue) or HP (orange) 28F3:B4 cells following staining with IgG controls, or antibodies directed against A) CD271, B) ABCB5 (surface), C) ABCB5 (intracellular), D) SOX10, E) JARID1B, F) A2B5, and G) GD2. Background fluorescence was determined separately for LP and HP cells using IgG controls. % of cells indicated in each positive gate.

Article Snippet: Primary antibodies were: anti-CD271-PE (Miltenyi, #120-002-227), anti-c-Kit-PE (eBioscience, #12-1179-41), anti-CD49f-FITC (BD Biosciences, #555735), anti-ABCB5 (Sigma, #HPA026975), anti-ALDH1A1 (Abcam, #ab52492), anti-L1CAM (Abcam, #ab24345), anti-CD51 (BD Biosciences, #611013), anti-A2B5 (Invitrogen, #433110) and anti-GD2 (BD Biosciences, #554272).

Techniques: Staining, Fluorescence

Journal: International Journal of Nanomedicine

Article Title: The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

doi: 10.2147/IJN.S116171

Figure Lengend Snippet: Changes in markers expression during iPSC-NPs differentiation detected using flow cytometry

Article Snippet: To analyze the human iPSC-NPs, conjugated antibodies against CD15 (SSEA-1)/PerCP, CD24/FITC, CD29/APC, CD44/FITC, CD56 (NCAM)/APC or PE, CD271 (NGFR)/PE (Exbio Antibodies, Vestec u Prahy, Czech Republic), Ki67/FITC, sox2/APC (BD Pharmingen, San Diego, CA, USA), SSEA4/PE (BioLegend, San Diego, CA, USA), nanog/FITC, oct3/4/APC, TRA-1-60/PE, CD184/PE (eBioscience, San Diego, CA, USA), A2B5/APC, CD133/PE (Miltenyi Biotec, Bergisch Gladbach, Germany) and β-III-tubulin/FITC (BD Biosciences) were used.

Techniques: Expressing, Labeling